Canine Hypothyroidism:
Prevalence Of Positive TgAA in 105 Laboratory Samples From Giant Schnauzers

By RF Nachreiner, M Bowman, KR Refsal PA Graham, A Provencher Bolliger Animal Health Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing, MI
Reprinted from Giant Steps July-Aug. 2000
 

Hypothyroidism is the most common endocrine disease to dogs. The thyroid gland controls the speed of metabolism of almost all body cells. When thyroid hormones are subnormal, many different body systems can be affected; so the clinical appearance of the disease can vary. The most common clinical signs include: weight gain, sluggishness, skin and hair coat problems (including hair loss), weakness, cold intolerance, and infertility. Although hypothyroidism is not life threatening, the quality of life is subnormal. Veterinary testing procedures have improved over the past 25 years, and hypothyroidism and its therapy are well understood by most practitioners. Breeders, however, are faced with a dilemma. The disease seldom demonstrates clinical signs before 3 to 5 years of age, well into the showing and breeding years for many dogs. An economical and early detection procedure is needed. Progress is being made toward this objective. 

The 1997 AKC Parent Club Survey found that breeders are quite concerned about this disease. In fact, it was ranked first, with hip dysplasia and epilepsy close behind. As a result of breeder and veterinary interest, a number of progressive changes have occurred in the past few years. AKC and other groups sponsored an International Symposium on Canine Hypothyroidism. Participants agreed that breeders should test their dogs for thyroid disease and this test profile should include Total Thyroxine (T4), Thyroid Stimulating Hormone (TSH), Free T4 by Dialysis, and Thyroglobulin Autoantibody (TgAA). The Orthopedic Foundation for Animals (OFA) started a Canine Thyroid Registry and has certified a number of regional veterinary laboratories in the US and Canada qualified to perform the thyroid profile for registry purposes. Oxford Laboratories began producing a commercially available assay for TgAA so all reagents for the OFA profile is standard among certified laboratories. 

About half of canine hypothyroidism has been reported to be associated with autoimmune thyroiditis (positive TgAA). The majority of the remaining hypothyroidism is idiopathic (without apparent cause and TgAA negative), while a small fraction is from a pituitary disorder. Recent data from Michigan State University have shown that idiopathic hypothyroidism can be the end stage of autoimmune thyroid disease. Hence, the majority of canine hypothyroidism is the result of autoimmune thyroiditis. This makes testing for the disease marker in breeding dogs important for reducing its incidence. The best current marker for this disease is a positive TgAA test result. This is present when there is active thyroid disease (inflammation). Assuming a single gene disorder and recessive trait, TgAA will be positive only in dogs having both genes for thyroiditis. TgAA will not be positive in the carriers (having only one gene for the trait) and may not be positive early in the life of some dogs that become affected later. 

A number of scientific publications have presented data to support the genetic transmission of autoimmune thyroiditis. Others have reported the familial (occurring among relatives) incidence of the disease. In addition to screening breeding animals, the TgAA assay has been useful for disease diagnosis. In June 1998, the Michigan State University Animal Health Diagnostic laboratory began running the TgAA assay as part of routine thyroid function testing, making it possible to detect autoimmune thyroid disease long before complete thyroid atrophy and clinical hypothyroidism (when clinical signs are present) occurred. Of course, most of the samples were from dogs with at least one clinical sign suggestive of hypothyroidism. 

A study done on the first 51,201 laboratory samples that were tested for thyroid disease using the new TgAA test indicated that 7.9% of samples were positive and 4.0% were inconclusive. There were 4045 dogs positive for TgAA and 2809 classified as having idiopathic hypothyroidism. Dogs less than 2 years of age had few (less than 5%a) TgAA positive samples; samples from dogs 2 to 6 years of age were positive 9 to 11.5% of the time, while samples from dogs older than 6 were positive less often. It appears that autoimmune thyroid disease occurs earlier in life than idiopathic hypothyroidism, supporting the concept that the idiopathic form may result from the autoimmume disease. Semiannual thyroid biopsy results from a small group of affected dogs at MSU also support the conclusion that idiopathic hypothyroidism is the end stage of autoimmune thyroid disease. 

Another way of looking at these data is that over 50% of TgAA positive samples were from dogs less than or equal to 5 years of age, while it took 8 years to obtain more than 50% of the samples from dogs with idiopathic hyporhyroidism.  Since idiopathic hypothyroidism appears to be the end stage of autoimmune thyroiditis, the majority of primary hypothyroidism in dogs is most likely a result of the autoimmune disease. An important fact for breeders is that TgAA test can detect this disease years before clinical signs of hypothyroidism occur. After 6 years, the TgAA test becomes less relevant as dogs that were positive can become negative when the thyroid is destroyed, and there is no longer a stimulus for TgAA production. After that time, T4 and TSH become more important indicators of thyroid disease. 

As one would expect from a genetically transmitted disease, 16 breeds had significantly higher laboratory prevalence of TgAA compared to the total number of dogs (over‑represented) and 27 breeds had a significantly lower prevalence (under‑represented)(Table 1). Some additional breeds also had high or low percent positive, but the number of samples from those breeds may have been too small to achieve statistical significance. As additional samples are analyzed, presumably these additional breeds will become statistically significant as well. 

As groups, Herding and Sporting dogs were significantly over‑represented for TgAA positive samples while Hounds, Non‑Sporting dogs, Terriers, and Toy breeds were under-represented. Within those groups, some breeds were significantly over‑represented while others may be under‑represented. The Working Group (Table 2) has 3 breeds with over 500 samples, 2 were similar to the average for the Working Group (Doberman Pinscher and Rottweiler) and 1 is over‑represented (Boxer). Though Boxers, along with Huskies, and Akitas were over‑represented, others were significantly underrepresented (Newfoundland Portuguese Water Dog and Schnauzer). Hence, the Working Group as a whole had an average prevalence that was similar to the all breed average. This is not to say that since the group is average, one should not be concerned about autoimmume thyroid disease. Since there is a genetic component to the disease, some breeds have a higher prevalence while others have less. One should strive to reduce the disease in the higher prevalence breeds and keep it low in the lower prevalence breeds. The Giant Schnauzer had 12.4% of the samples positive for autoimmune thyroid disease. This is higher than the all breed average, but was not statistically significant. As sample numbers increase, statistical significance may be achieved.

Breeders should strive to reduce the prevalence of autoimmune thyroiditis within the breed. Testing breeding animals for TgAA during their early reproductive years and breeding appropriately will help accomplish that objective. The assay can be performed on serum samples at most of the larger veterinary laboratories or on blood spots at Oxford Laboratories. 

In conclusion, while there is no DNA based testing procedure at this time, testing with the best available marker, TgAA, can be beneficial. Selective breeding should reduce the prevalence in high incidence breeds and prevent an increasing prevalence in low incidence breeds. Although males were similar to females in prevalence, you can imagine the impact one important but affected male could have on the breed, especially a breed with small numbers. The TgAA portion of the thyroid profile is most important during the first 5 years of life as few dogs are found to have idiopathic hypothyroidism before this age. Since idiopathic hypothyroidism appears to be the end stage of autoimmune thyroiditis, the majority of hypothyroidism in dogs is a result of this inherited autoimmune disease. An increased focus on testing for autoimmune thyroiditis and selective breeding should help decrease the prevalence of canine hypothyroidism. In addition, knowing which are affected dogs within a pedigree will help to identify which dogs have a high probability of being carriers (i.e. one affected gene if a single gene trait). 

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